Efficiency of nano-electrospray ionization
نویسندگان
چکیده
منابع مشابه
A hydrodynamically optimized nano-electrospray ionization source and vacuum interface.
The coupling of atmospheric pressure ionization (API) sources like electrospray ionization (ESI) to vacuum based applications like mass spectrometry (MS) or ion beam deposition (IBD) is done by differential pumping, starting with a capillary or pinhole inlet. Because of its low ion transfer efficiency the inlet represents a major bottleneck for these applications. Here we present a nano-ESI vac...
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We report a new microfluidic emitter for nano-electrospray ionization mass spectrometry (nanoESI-MS), made from polyimide tape. In contrast to the conventional pulled glass capillary emitters typically used for nanoESI-MS, these new polyimide emitters are simple and inexpensive to make, and are disposable after a single use. Thus, sample contamination and clogging are never a problem when analy...
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Lipids, such as phosphoinositides (PIPs) and diacylglycerol (DAG), are important signaling intermediates involved in cellular processes such as T cell receptor (TCR)-mediated signal transduction. Here we report identification and quantification of PIP, PIP2 and DAG from crude lipid extracts. Capitalizing on the different extraction properties of PIPs and DAGs allowed us to efficiently recover b...
متن کاملPrinciples of electrospray ionization.
Electrospray ionization is today the most widely used ionization technique in chemical and biochemical analysis. Interfaced with a mass spectrometer it allows the investigation of the molecular composition of liquid samples. With electrospray a large variety of chemical substances can be ionized. There is no limitation in mass which thus enables even the investigation of large noncovalent prote...
متن کاملIonization and transmission efficiency in an electrospray ionization-mass spectrometry interface.
The ionization and transmission efficiencies of an electrospray ionization (ESI) interface were investigated to advance the understanding of how these factors affect mass spectrometry (MS) sensitivity. In addition, the effects of the ES emitter distance to the inlet, solution flow rate, and inlet temperature were characterized. Quantitative measurements of ES current loss throughout the ESI int...
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ژورنال
عنوان ژورنال: Journal of the American Society for Mass Spectrometry
سال: 2005
ISSN: 1044-0305,1879-1123
DOI: 10.1016/j.jasms.2005.06.011